Stable Cell Line Generation Service

Stable cell lines are manufactured to generate constitutive overexpression or knockdown genes via the incorporation of transfected plasmid dna or reporter gene constructs into the genome.

Stable cell line generation service.

The transfection method determines the cell type for stable integration. Cell line development service options are available from several specialized providers. Stable expression can be influenced by the transfection method used. Stable cell line generation cellomics technology llc provides high quality fast turnaround and cost effective stable cell line generation service for research biotechnology and pharmaceutical companies.

This protocol can be used to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector. Stable cell line generation proteogenix strives to overcome the economic issues related to stable cell line generation by offering the most productive ip free cell lines on the market. Stable cell lines with specific gene over expression or knock down are very helpful in gene function analysis target discovery target vali. Major challenges for generation of stable cell lines are low transfection efficiency and or integration frequency.

Choosing to outsource this application may not only save time and effort but can also provide benefits from technical expertise and facilities dedicated to the generation of stable cell lines. Point mutation at a defined locus are widely employed in functional studies drug discovery assays and recombinant protein production. It is known that liposome reagents can be used to transfer dna into adherent cell lines. The development of stable cell lines is an important research tool in pharmaceutical drug discovery compound screening and gene therapy research.

Unlike the short term protein expression observed using transient transfection approaches generating cell lines using lentiviral vectors enables long term protein expression studies.