By applying single cell clonings from the selected stable pools selexis identifies high expressing clonal cell lines that are then evaluated for their.
Stable cell line development.
The development of high yield production cell lines starts with the cloning of the gene of interest into sure tech vectors that bear selexis sge selexis genetic elements.
Foreign dna encoding the protein of interest often includes an additional selectable marker.
One is achieved by eukaryotic vectors that harbor elements for episomal maintenance in the nucleus of a transfected cell.
Proteogenix strives to overcome the economic issues related to stable cell line generation by offering the most productive ip free cell lines on the market.
Virtually any cdna mab and multigenic applications can be packaged into the gpex retrovector and used to insert your gene of interest into cells.
Stable expression of target gene in cell lines overcomes the low transfection efficiency of transient expression and produces more target proteins.
The jump in technology helps accelerate stable cell line development due to the targeted integration of genetic material into specific integration sites using phic31 integrase.
Typical cell line workflow step 1.
The process of cell line development begins with the introduction of foreign dna encoding.
This protocol describes the procedure for creating a stable cell line based on the expicho expression system for use in commercial bioproduction.
With a one time fee and strong yield guarantees our stable cell line development service based on our proprietary cell line aims at offering high productivity at competitive price and fto without royalties.
Gpex cell line development technology is a proven technology that generates high performance highly stable production cell lines.
There are two types of stable cell lines.
Stable cell line generation.
You want to develop a stable cell line because it is stable in expressing the target protein.
This is a very common problem for many stable cell lines.
Stable cell line development cell engineering group offers services for cell line development from dna sequences to rcb stability assessment.
To date over 600 different mab and mab fusions and over 80 different recombinant proteins have been produced using the gpex system with 120 ongoing clinical trials utilizing therapeutic candidates developed.
That s why gibco products are designed to provide you with integrated stable cell line development solutions to simplify your development workflow and accelerate your processes to commercial manufacture.
However not all stable cell lines are equally stable.
We understand that cell line development involves multiple complex processes requiring consistency and reproducible results.
After several passages some stable cell lines may become unstable with their expression diminishing slowly.
